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北京时间10月5日17时45分，瑞典皇家科学院宣布将2022年诺贝尔化学奖授予美国化学家卡罗林·贝尔托西（Carolyn R. Bertozzi）、丹麦化学家摩顿·梅尔达尔Morten Meldal以及美国化学家巴瑞·夏普莱斯（K. Barry Sharpless），以表彰他们在点击化学和正交化学研究方面的贡献。继F.Sanger之后，Sharpless先生也两度成为诺贝尔化学奖的得主。2001年，诺贝尔奖的一半授予了Sharpless以表彰他在不对称催化氧化领域的贡献（另一半授予了William S. Knowles和Ryoji Noyori三位化学家以表彰他们在…
立春，二十四节气之首，立是开始，春是希望。冬去春来，万物渐次复苏。如期而至的不止是春天，还有疫情过后的平安喜乐。一年之计在于春！一元复始，万象更新。在每年的春天，都要做好人生的打算，选择好努力的方向，计划好奋斗的目标，愿你我不负春光，不负梦想！！立春后五日，春态纷婀娜。——白居易《立春后五日》
The copper-catalyzed azide-alkyne cycloaddition reaction, or CuAAC, has been used extensively for the conjugation, immobilization, and purification of biomolecules as well as for monitoring various biological processes. Recent advances in design of copper chelating ligands such as THTPA or BTTAA improved kinetics of copper-catalyzed azide-alkyne cycloaddition reaction and greatly increased sensitivity of alkyne detection. In spite of many recent …
Figure 1). Kinetic comparison of chelating azide and non-chelating conventional azideRecent advances in the design of copper-chelating ligands, such as THPTA or BTTAA that stabilize the Cu(I) oxidation state in aqueous solution, improve the kinetics of the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction and greatly increase the sensitivity of alkyne detection. Copper-chelating ligands have also been shown to increase the biocompatibi…
ClickChemistryTools专注于点击化学领域，致力于打造国际一流的专业领域品牌，提供全面的、创新的、优质的、便捷的科研产品和服务。目前，其高品质产品屡登国际权威期刊，并获得了国际学术界的认可，为进一步推动了品牌本土化的传播，靶点科技（北京）有限公司携手ClickChemistryTools在中国开展品牌和产品宣传的活动。为了感谢和回馈广大中国客户的支持，ClickChemistryTools特设产品文献推广计划，旨在建立产品应用数据库，为广大科研工作者提供产品使用的参考价值。靶点科技期待与您…
Click-&-Go IsoTAG Kit for Profiling Intact GlycopeptidesWhile there has been much interest in profiling the intact glycoproteome, the complexity of glycoproteoforms (and more broadly, all proteoforms) remains challenging to completely define. Mass spectrometry (MS) is commonly employed for characterization of complex proteomic samples. A popular strategy for protein identification is the bottom-up shotgun proteomics approach. In this method, …
Protein Labeling Calculator在线工具
Metabolic Labeling of Glycoproteins 下载为PDFGeneral Information
This protocol details the preparation of cell lysates metabolically labeled with azido- or alkynylsugar. However, investigators may wish to determine the optimal concentration of metabolic
reagent as well as labeling time individually for metabolic labeling for…
Metabolic Labeling with AHA or HPG 下载为PDFGeneral Information For imaging, culture cells on cover slips or other desired imaging-compatible surface,
approximately 1 × 106 cells are sufficient for one detection. 1 × 106 cells are required to prepare
100 μg of lysate. Metabolic Labeling of Cells The following is a standa…
Labeling cells with EdU 下载为PDFThe following is a general protocol was tested with several cell lines including A549, HeLa, and NIH/3T3
cells, but it can be used for any adherent cell type. Growth medium, cell density, cell type variations, and
other factors may influence labeling. We recommend 10 μM E…
EdU Imaging, Flow Cytometry 下载为PDFThis protocol was tested with A549, HeLa, and NIH/3T3 cells but it can be adapted for any adherent cell
type. In initial experiments, we recommend starting with 1.5 µM final concentration of an azide detection
reagent. Slight adjustment of an azide detection reagent concentration might be r…
EdU Imaging, Microscopy 下载为PDFThis protocol was tested with A549, HeLa, and NIH/3T3 cells but it can be adapted for any adherent cell
type. In initial experiments, we recommend starting with 5 µM concentration of an azide detection
reagent. Slight adjustment of an azide detection reagent concentration might be r…
Cell Lysate Labeling 下载为PDF This is a general protocol for labeling cell lysate through copper-catalyzed click reaction with any azide or
alkyne click detection reagent. This protocol may be used as a starting point for optimization of particular
click c…
TCO – Tetrazine Ligation(Figure 1). Schematic representation of a TCO–Tz ligation reaction.At low biomolecule concentrations (e.g. < 5 μM) where SPAAC falls short due to modest kinetics (e.g. 0.3 to 2.3 M–1s–1), and where copper–catalyzed alkyne–azide cycloaddition click reaction might compromise system viability, trans–cyclooctene–tetrazine (TCO–Tz) is the reaction pair of choice. This ligation chemistry is based on an inverse–dema…
Cu – Free Click Chemistry (SPAAC)(Figure 1). Schematic representation of a SPAAC ligation reaction.The strain promoted alkyne-azide cycloaddition, also termed as the Cu-free click reaction, is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as amines (Figure 1). SPAAC enables labeling a wide varie…
Click Chemistry Tools（CCT）位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ)，是一家创新的生物技术研究公司，专注于点击化学（Click Chemistry）领域的产品研发和生产。点击化学（ Click chemistry）是2001年，美国诺贝尔化学奖获得者、史格堡研究院（Skaggs institute）化学生物研究所的研究员贝瑞夏普利斯（K. Barry Sharpless）发展出一种新技术，其所具有的高效和高控制性。点击化学的基本思想就是利用碳-杂原子成键反应快速实现分子多样性，一般由叠氮化物（azide）和炔烃（alk…
