代谢标记技术

用叠氮基或炔基糖代谢标记细胞裂解物的制备Protocol
Metabolic Labeling of Glycoproteins 下载为PDFGeneral Information This protocol details the preparation of cell lysates metabolically labeled with azido- or alkynylsugar. However, investigators may wish to determine the optimal concentration of metabolic reagent as well as labeling time individually for metabolic labeling for…
用于制备AHA或HPG标记的细胞裂解液的Protocol
Metabolic Labeling with AHA or HPG 下载为PDFGeneral Information For imaging, culture cells on cover slips or other desired imaging-compatible surface, approximately 1 × 106 cells are sufficient for one detection. 1 × 106 cells are required to prepare 100 μg of lysate. Metabolic Labeling of Cells The following is a standa…