点击化学Protocols

Labeling cells with EdU 下载为PDFThe following is a general protocol was tested with several cell lines including A549, HeLa, and NIH/3T3 cells, but it can be used for any adherent cell type. Growth medium, cell density, cell type variations, and other factors may influence labeling. We recommend 10 μM E…

EdU Imaging, Flow Cytometry 下载为PDFThis protocol was tested with A549, HeLa, and NIH/3T3 cells but it can be adapted for any adherent cell type. In initial experiments, we recommend starting with 1.5 µM final concentration of an azide detection reagent. Slight adjustment of an azide detection reagent concentration might be r…

EdU Imaging, Microscopy 下载为PDFThis protocol was tested with A549, HeLa, and NIH/3T3 cells but it can be adapted for any adherent cell type. In initial experiments, we recommend starting with 5 µM concentration of an azide detection reagent. Slight adjustment of an azide detection reagent concentration might be r…

Cell Lysate Labeling 下载为PDF This is a general protocol for labeling cell lysate through copper-catalyzed click reaction with any azide or alkyne click detection reagent. This protocol may be used as a starting point for optimization of particular click c…