Cy5 DBCO

A130-1

1 mg

2580.0

ClickChemistryTools

现询

A130-5

5 mg

8500.0

ClickChemistryTools

现询

A130-25

25 mg

23900.0

ClickChemistryTools

现询

A130-100

100 mg

59000.0

ClickChemistryTools

现询

Abs/Em Maxima: 649/671 nm

Extinction Coefficient: 250,000

Flow Cytometry Laser Line: 633 or 635 nm

Microscopy Laser Line: 633 or 635 nm

Spectrally Similar Dyes: Alexa Fluor® 647, CF™ 647 Dye, DyLight™649

Cy5

Cy5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

分子量

1009.22

分子式

N/A

CAS

1564286-24-3

溶(解)度

Water, DMSO, DMF

纯度

>95% (HPLC)

外观

Blue solid

储存环境

-20°C. Desiccate

运输条件

Ambient temperature

1. Bazrafshan, A. et al. (2021). DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second. ACS Publications, Online ahead of print. [PubMed]

2. Wiener, J., et al. (2020). Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics. Sci Rep., 10 (1), 1457. [PubMed]

3. Yong, K.W., et al. (2020). Engineering the Orientation, Density, and Flexibility of Single-Domain Antibodies on Nanoparticles To Improve Cell Targeting. ACS Appl. Mater. Interfaces, 12(5), 5593-600. [PubMed]

4. Song, S., et al. (2020). In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjugate Chem., 31(5), 1562-74. [PubMed]

5. Valentini, T.V., et al. (2020). Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota. Nat Commun., 11, 2287-74. [PMC]

6. Kim, S. H., et al. (2014). Cell labeling and tracking method without distorted signals by phagocytosis of macrophages . Theranostics, 4 (4), 420-31. [PubMed]

CoA Lot 2218