AFDye 488 Azide

货号
规格
价格
品牌
货期
1275-1
1 mg
3580.0
ClickChemistryTools
现询
1275-5
5 mg
10400.0
ClickChemistryTools
现询
1275-25
25 mg
32240.0
ClickChemistryTools
现询

Abs/Em Maxima: 494/517 nm

Extinction Coefficient: 73.000

Flow Cytometry Laser Line: 488 nm

Microscopy Laser Line: 488 nm

Spectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488

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AZDye™ 488 Azide (Alexa Fluor® 488 Azide equivalent) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 488 Azide, an equivalent of Alexa Fluor 488® Azide, is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A. AZDye™ 488 Azide can be used a less expensive alternative to Alexa Fluor® 488 Azide.


分子量
658.66 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Orange to light red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperature

1. Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms, 9, 1578. [MDPI]

2. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci., 135 (5), jcs258566. [PubMed]

3. Baskin, J. A., et al. (2021). A chemoproteomics approach to profile phospholipase D-derived phosphatidyl alcohol interactions. Cambridge: Cambridge Open Engage, This content is a preprint and has not been peer-reviewed. [ChemRxiv.]


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