AFDye 488 Azide

1275-1

1 mg

3580.0

ClickChemistryTools

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1275-5

5 mg

10400.0

ClickChemistryTools

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1275-25

25 mg

32240.0

ClickChemistryTools

现询

Abs/Em Maxima: 494/517 nm

Extinction Coefficient: 73.000

Flow Cytometry Laser Line: 488 nm

Microscopy Laser Line: 488 nm

Spectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488

AZDye™ 488 Azide (Alexa Fluor® 488 Azide equivalent) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 488 Azide, an equivalent of Alexa Fluor 488® Azide, is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A. AZDye™ 488 Azide can be used a less expensive alternative to Alexa Fluor® 488 Azide.

分子量

658.66 (protonated)

分子式

N/A

CAS

N/A

溶(解)度

Water, DMSO, DMF

纯度

>95% (HPLC)

外观

Orange to light red solid

储存环境

-20°C. Desiccate

运输条件

Ambient temperature

1. Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms, 9, 1578. [MDPI]

2. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci., 135 (5), jcs258566. [PubMed]

3. Baskin, J. A., et al. (2021). A chemoproteomics approach to profile phospholipase D-derived phosphatidyl alcohol interactions. Cambridge: Cambridge Open Engage, This content is a preprint and has not been peer-reviewed. [ChemRxiv.]