AFDye 430 Azide
Abs/Em Maxima: 430/537 nm
Extinction Coefficient: 15,000
Spectrally Similar Dyes: Alexa Fluor® 430, CF® 430
AZDye™ 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.
AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click react…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Azide is a water-soluble, pH-insensiti…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 Alkyne (Alexa Fluor® 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe that reacts with azid…
Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor® 430, CF® 430AZDye™ 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not re…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Alkyne reacts with azides via a copper…
Abs/Em Maxima: 494/517 nmExtinction Coefficient: 73.000Flow Cytometry Laser Line: 488 nmMicroscopy Laser Line: 488 nmSpectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488AZDye™ 488 Azide (Alexa Fluor® 488 Azi…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405ZDye™ 405 DBCO reacts with azides via a copper-fr…
