AFDye 488 DBCO
Abs/Em Maxima: 494/517 nm
Extinction Coefficient: 73,000
Flow Cytometry Laser Line: 488 nm
Microscopy Laser Line: 488 nm
CF260: 0.35
CF280: 0.19
Spectrally Similar Dyes: FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
AZDye™ 488 DBCO (Alexa Fluor® 488 DIBO analog) is a water-soluble, green-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AZDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
1. Simon P. Wisnovsky, et al. (2020). Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation. PNAS, 117 (41), 25293-25301. [PNAS]
2. Loebel, C., et al. (2020). Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte–Hydrogel Interface. Adv. Funct. Mater. [PubMed]
3. Loebel, C., et al. (2019). Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels. Nature Materials, 18, 883-891. [PubMed]
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click react…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Azide is a water-soluble, pH-insensiti…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 Alkyne (Alexa Fluor® 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe that reacts with azid…
Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor® 430, CF® 430AZDye™ 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not re…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Alkyne reacts with azides via a copper…
Abs/Em Maxima: 494/517 nmExtinction Coefficient: 73.000Flow Cytometry Laser Line: 488 nmMicroscopy Laser Line: 488 nmSpectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488AZDye™ 488 Azide (Alexa Fluor® 488 Azi…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405ZDye™ 405 DBCO reacts with azides via a copper-fr…
