AFDye 660R Tetrazine

1504-1

1 mg

3580.0

ClickChemistryTools

现询

1504-5

5 mg

11900.0

ClickChemistryTools

现询

1504-25

25 mg

35600.0

ClickChemistryTools

现询

Abs/Em Maxima: 665/690 nm

Extinction Coefficient: 92,000

Flow Cytometry Laser Line: 633 or 635 nm

Microscopy Laser Line: 633 or 635 nm

Spectrally Similar Dyes: Alexa Fluor® 660, CF® 660R

Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

AZDye 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. AZDye 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AZDye 660R make the dye an ideal choice for confocal microscopy and other demanding applications.

AZDye660_graph

AZDye 660R dye spectrally is almost identical to Alexa Fluor® 660 and CF® 660R Dye and can be used a less expensive alternative to these dyes.

分子量

913.05 (protonated)

分子式

N/A

CAS

N/A

溶(解)度

Water, DMSO, DMF

纯度

>95% (HPLC)

外观

Blue solid

储存环境

-20°C. Desiccate

运输条件

Ambient temperature

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]