AFDye 594 Tetrazine

货号
规格
价格
品牌
货期
1364-1
1 mg
3580.0
ClickChemistryTools
现询
1364-5
5 mg
13900.0
ClickChemistryTools
现询
1364-25
25 mg
43000.0
ClickChemistryTools
现询

Abs/Em Maxima: 590/617 nm

Extinction Coefficient: 92,000

Flow Cytometry Laser Line: 561 or 594 nm

Microscopy Laser Line: 561 or 594 nm

Spectrally Similar Dyes: Alexa Fluor® 594, Atto™ 594, CF™ 594 Dye, DyLight™594

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Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

AZDye™ 594 (Alexa Fluor® 594 equivalent) is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AZDye™ 594 is structurally identical to Alexa Fluor® 594. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including DyLight® 594, Alexa Fluor® 594, and CF® 594 Dye.

分子量
892.98
分子式
N/A
CAS
N/A
溶(解)度
Water, MeOH, DMSO, DMF
纯度
>95% (HPLC)
外观
Red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperature

1. Marquard, A.N., et al. (2020). Expanding the Scope of Antibody Rebridging with New Pyridazinedione-TCO Constructs. Bioconjug Chem., 31(6), 1616-23. [PubMed]


                 

CoA Lot 2432    


                       

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