AFDye 488 Tetrazine
Abs/Em Maxima: 493/517 nm
Extinction Coefficient: 83,000
Flow Cytometry Laser Line: 488 nm
Microscopy Laser Line: 488 nm
Spectrally Similar Dyes: Alexa Fluor® 488, Atto™ 488, CF™ 488 Dye, DyLight™488
Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.
AZDye™ 488 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 493 and 517 nm, respectively. It can be used with the 488 nm laser line. AZDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488.
DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific. Click Chemistry Tools is not affiliated with or endorsed by Thermo Fisher Scientific. AZDye™ 488 is NOT manufactured, distributed, or affiliated in any way with Thermo Fisher Scientific.
1. Marquard, A.N., et al. (2020). Expanding the Scope of Antibody Rebridging with New Pyridazinedione-TCO Constructs. Bioconjug Chem., 31(6), 1616-23. [PubMed]
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Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor® 430, CF® 430AZDye™ 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
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