AFDye 532 Azide
Abs/Em Maxima: 532/554 nm
Extinction Coefficient: 78,000
Flow Cytometry Laser Line: 532 nm
Microscopy Laser Line: 532 nm
Spectrally Similar Dyes: Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
AZDye™ 532 Azide (Alexa Fluor® 532 equivalent) is a bright, yellow-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 532 Azide is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.
AZDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click react…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Azide is a water-soluble, pH-insensiti…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 Alkyne (Alexa Fluor® 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe that reacts with azid…
Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor® 430, CF® 430AZDye™ 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not re…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Alkyne reacts with azides via a copper…
Abs/Em Maxima: 494/517 nmExtinction Coefficient: 73.000Flow Cytometry Laser Line: 488 nmMicroscopy Laser Line: 488 nmSpectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488AZDye™ 488 Azide (Alexa Fluor® 488 Azi…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405ZDye™ 405 DBCO reacts with azides via a copper-fr…
