Cy3 Picolyl Azide

1178-1

1 mg

3580.0

ClickChemistryTools

现询

1178-5

5 mg

10900.0

ClickChemistryTools

现询

1178-25

25 mg

31800.0

ClickChemistryTools

现询

Abs/Em Maxima: 555/572nm

Extinction Coefficient: 150,000

Flow Cytometry Laser Line: 532 or 555 nm

Microscopy Laser Line: 532 or 555 nm

Spectrally Similar Dyes: Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549, Cy3 Dye

Cy3 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

Cy3 Picolyl Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 dye is structurally similar, and spectrally is almost identical to Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

分子量

927.08 (protonated)

分子式

N/A

CAS

N/A

溶(解)度

Water, DMSO, DMF

纯度

>95% (HPLC)

外观

Red solid

储存环境

-20°C. Desiccate

运输条件

Ambient temperature

1. Couradeau, E., et al. (2019). Probing the active fraction of soil microbiomes using BONCAT-FACS Nat Commun, 10, 2770-80. [PubMed]

2. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

3. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

4. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]