AFDye 546 Picolyl Azide

1284-1

1 mg

3580.0

ClickChemistryTools

现询

1284-5

5 mg

10980.0

ClickChemistryTools

现询

1284-25

25 mg

30700.0

ClickChemistryTools

现询

Abs/Em Maxima: 554/570 nm

Extinction Coefficient: 110,000

Flow Cytometry Laser Line: 532 nm

Microscopy Laser Line: 543 nm or 546 nm

Spectrally Similar Dyes: Alexa Fluor^® 546, Atto™ 546, CF^® 546

AZDye™ 546 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

AZDye™ 546 (Alexa Fluor® 546 equivalent) is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AZDye™ 546 dye is structural and spectrally identical Alexa Fluor® 546.

分子量

1050.39 (protonated)

分子式

N/A

CAS

N/A

溶(解)度

Water, DMSO, DMF

纯度

>95% (HPLC)

外观

Red solid

储存环境

-20°C. Desiccate

运输条件

Ambient temperature

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]