AFDye 488 Alkyne

货号
规格
价格
品牌
货期
1277-1
1 mg
3180.0
ClickChemistryTools
现询
1277-5
5 mg
9900.0
ClickChemistryTools
现询
1277-25
25 mg
30700.0
ClickChemistryTools
现询

Abs/Em Maxima: 494/517 nm

Extinction Coefficient: 73,000

Flow Cytometry Laser Line: 488 nm

Microscopy Laser Line: 488 nm

Spectrally Similar Dyes: FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488

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AZDye™ 488 Alkyne (Alexa Fluor® 488 Alkyne equivalent) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AZDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

A probe for copper-less azide detection (AZDye™ 488 DBCO) is also available for application where the presence of copper is not acceptable.

AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.

AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A. AZDye™ 488 Alkyne can be used a less expensive alternative to Alexa Fluor® 488 Alkyne.

分子量
571.53 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Orange to light red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperature

1. Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms, 9, 1578. [MDPI]


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